Practical HPLC method development / Lloyd R. Snyder, Joseph J. Kirkland, Joseph L. Glajch.
Τύπος υλικού:
ΚείμενοΛεπτομέρειες δημοσίευσης: New York : Wiley, �1997.Έκδοση: 2nd edΠεριγραφή: 1 online resource (xxvi, 765 pages) : illustrationsΤύπος περιεχομένου: - text
- computer
- online resource
- 0585301115
- 9780585301112
- 9781118592014
- 1118592018
- 9780471007036
- 047100703X
- 9781118591512
- 1118591518
- High performance liquid chromatography -- Methodology
- Chromatographie en phase liquide �a hautes performances -- M�ethodologie
- SCIENCE -- Chemistry -- Analytic
- Chromatographie
- Analyse chimique
- M�ethodes de recherche
- High performance liquid chromatography -- Methodology
- Cromatografia em liquido
- Chimie -- Manipulation
- Chromatographie en phase liquide �a hautes performances -- M�ethodologie
- Chromatography, High Pressure Liquid -- methods
- 543/.0894 20
- QP519.9.H53 S69 1997eb
- QD 79 .C454 S675p 1997
- 35.70
"A Wiley-Interscience publication."
Includes bibliographical references and index.
Print version record.
1. Getting Started -- 2. Basics of Separation -- 3. Detection Sensitivity and Selectivity -- 4. Sample Preparation -- 5. Column -- 6. Non-ionic Samples: Reversed- and Normal-Phase HPLC -- 7. Ionic Samples: Reversed-Phase, Ion-Pair, and Ion-Exchange HPLC -- 8. Gradient Elution -- 9. Systematic Approach to the Reversed-Phase Separation of Regular Samples -- 10. Computer-Assisted Method Development -- 11. Biochemical Samples: Proteins, Nucleic Acids, Carbohydrates, and Related Compounds -- 12. Chiral Separations -- 13. Preparative HPLC Separation -- 14. Quantitation (Including Trace Analysis) -- 15. Completing the Method: Validation and Transfer -- App. I. Plate Number and Resolution -- App. II. Properties of Solvents Used in HPLC -- App. III. Retention in Reversed-Phase and Normal-Phase HPLC as a Function of Sample Molecular Structure -- App. IV. Preparing Buffered Mobile Phases -- App. V. Characterizing the Differences Among C[subscript 8] or C[subscript 18] Reversed-Phase Columns from Different Suppliers -- App. VI. Adjusting Mobile-Phase Water Content for Normal-Phase HPLC.
Thoroughly revised and greatly expanded, the Second Edition of this landmark work brings scientists completely up to date on method development for HPLC, one of the main analytical techniques in use today. The authors, widely recognized as preeminent authorities in the field, offer the most far-reaching treatment available, taking into account advances in chromatography and the increasingly complex sample types that have become significant in the past decade.
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