Practical HPLC method development /
Snyder, Lloyd R.
Practical HPLC method development / Lloyd R. Snyder, Joseph J. Kirkland, Joseph L. Glajch. - 2nd ed. - New York : Wiley, �1997. - 1 online resource (xxvi, 765 pages) : illustrations
"A Wiley-Interscience publication."
Includes bibliographical references and index.
Getting Started -- Basics of Separation -- Detection Sensitivity and Selectivity -- Sample Preparation -- Column -- Non-ionic Samples: Reversed- and Normal-Phase HPLC -- Ionic Samples: Reversed-Phase, Ion-Pair, and Ion-Exchange HPLC -- Gradient Elution -- Systematic Approach to the Reversed-Phase Separation of Regular Samples -- Computer-Assisted Method Development -- Biochemical Samples: Proteins, Nucleic Acids, Carbohydrates, and Related Compounds -- Chiral Separations -- Preparative HPLC Separation -- Quantitation (Including Trace Analysis) -- Completing the Method: Validation and Transfer -- Plate Number and Resolution -- Properties of Solvents Used in HPLC -- Retention in Reversed-Phase and Normal-Phase HPLC as a Function of Sample Molecular Structure -- Preparing Buffered Mobile Phases -- Characterizing the Differences Among C[subscript 8] or C[subscript 18] Reversed-Phase Columns from Different Suppliers -- Adjusting Mobile-Phase Water Content for Normal-Phase HPLC. 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. App. I. App. II. App. III. App. IV. App. V. App. VI.
Thoroughly revised and greatly expanded, the Second Edition of this landmark work brings scientists completely up to date on method development for HPLC, one of the main analytical techniques in use today. The authors, widely recognized as preeminent authorities in the field, offer the most far-reaching treatment available, taking into account advances in chromatography and the increasingly complex sample types that have become significant in the past decade.
0585301115 9780585301112 9781118592014 1118592018 9780471007036 047100703X 9781118591512 1118591518
High performance liquid chromatography--Methodology.
Chromatographie en phase liquide �a hautes performances--M�ethodologie.
SCIENCE--Chemistry--Analytic.
Chromatographie.
Analyse chimique.
M�ethodes de recherche.
High performance liquid chromatography--Methodology.
Cromatografia em liquido.
Chimie--Manipulation.
Chromatographie en phase liquide �a hautes performances--M�ethodologie.
Chromatography, High Pressure Liquid--methods.
Electronic books.
QP519.9.H53 / S69 1997eb
543/.0894
QD 79 .C454 S675p 1997
Practical HPLC method development / Lloyd R. Snyder, Joseph J. Kirkland, Joseph L. Glajch. - 2nd ed. - New York : Wiley, �1997. - 1 online resource (xxvi, 765 pages) : illustrations
"A Wiley-Interscience publication."
Includes bibliographical references and index.
Getting Started -- Basics of Separation -- Detection Sensitivity and Selectivity -- Sample Preparation -- Column -- Non-ionic Samples: Reversed- and Normal-Phase HPLC -- Ionic Samples: Reversed-Phase, Ion-Pair, and Ion-Exchange HPLC -- Gradient Elution -- Systematic Approach to the Reversed-Phase Separation of Regular Samples -- Computer-Assisted Method Development -- Biochemical Samples: Proteins, Nucleic Acids, Carbohydrates, and Related Compounds -- Chiral Separations -- Preparative HPLC Separation -- Quantitation (Including Trace Analysis) -- Completing the Method: Validation and Transfer -- Plate Number and Resolution -- Properties of Solvents Used in HPLC -- Retention in Reversed-Phase and Normal-Phase HPLC as a Function of Sample Molecular Structure -- Preparing Buffered Mobile Phases -- Characterizing the Differences Among C[subscript 8] or C[subscript 18] Reversed-Phase Columns from Different Suppliers -- Adjusting Mobile-Phase Water Content for Normal-Phase HPLC. 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. App. I. App. II. App. III. App. IV. App. V. App. VI.
Thoroughly revised and greatly expanded, the Second Edition of this landmark work brings scientists completely up to date on method development for HPLC, one of the main analytical techniques in use today. The authors, widely recognized as preeminent authorities in the field, offer the most far-reaching treatment available, taking into account advances in chromatography and the increasingly complex sample types that have become significant in the past decade.
0585301115 9780585301112 9781118592014 1118592018 9780471007036 047100703X 9781118591512 1118591518
High performance liquid chromatography--Methodology.
Chromatographie en phase liquide �a hautes performances--M�ethodologie.
SCIENCE--Chemistry--Analytic.
Chromatographie.
Analyse chimique.
M�ethodes de recherche.
High performance liquid chromatography--Methodology.
Cromatografia em liquido.
Chimie--Manipulation.
Chromatographie en phase liquide �a hautes performances--M�ethodologie.
Chromatography, High Pressure Liquid--methods.
Electronic books.
QP519.9.H53 / S69 1997eb
543/.0894
QD 79 .C454 S675p 1997
