Protein localization by fluorescence microscopy : a practical approach / edited by Victoria J. Allan.

Includes bibliographical references and index.

Print version record.

There is an ever-increasing number of genes that have been sequenced but are of completely unknown function. The ability to determine the location of such gene products within the cell, either by the use of antibodies or by the production of chimeras with green fluorescent protein, is a vital step towards understanding what they do. This is one major reason why fluorescence microscopy is enjoying a revival. This no-nonsense guide provides detailed, practical advice on all aspects of the subject: from choosing the right equipment, to interpreting results. It balances the advantages of a wide range of techniques - including live cell work - against the potential pitfalls, offering invaluable "tricks of the trade" along the way. Protein Localization by Fluorescence Light Microscopy: A Practical Approach has something to offer all microscopists, giving a solid grounding to the novice whilst extending the range of the experienced user.

Machine generated contents note: 1. Basic immunofluorescence / Victoria J. Allan -- 2. Immunofluorescent labelling of sections / J. Victor Small / Alison J. North -- 3. Simultaneous in situ detection of DNA and proteins / Klaus Ersfeld / Elisa M. Stone -- 4. Instruments for fluorescence imaging / W. B. Amos -- 5. Fluorescence microscopy of living vertebrate cells / David Shima / Rainer Pepperkok -- 6. Visualizing fluorescence in Drosophila -- optimal detection in thick specimens / Ilan Davis -- 7. Green fluorescent protein in plants / Chris Hawes / Petra Boevink / Ian Moore -- 8. Fluorescence microscopy in yeast / Iain M. Hagan / Kathryn R. Ayscough -- App. A1 Excitation and emission maxima of commonly used fluorophores -- App. A2 Commercial sources of antibodies -- App. A3 Optical units -- App. A4 List of Suppliers.

OCLC WorldCat Holdings

eBooks on EBSCOhost All EBSCO eBooks